Partial and Full PCR-Based Reverse Genetics Strategy for Influenza Viruses
نویسندگان
چکیده
منابع مشابه
Partial and Full PCR-Based Reverse Genetics Strategy for Influenza Viruses
Since 1999, plasmid-based reverse genetics (RG) systems have revolutionized the way influenza viruses are studied. However, it is not unusual to encounter cloning difficulties for one or more influenza genes while attempting to recover virus de novo. To overcome some of these shortcomings we sought to develop partial or full plasmid-free RG systems. The influenza gene of choice is assembled int...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2012
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0046378